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Bioss rabbit anti peg3
Oligonucleotides and annealing temperature utilized for qAMP and qRT-PCR of imprinted genes

Rabbit Anti Peg3, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+peg3/pmc03896855-75-11-14?v=Bioss
Average 92 stars, based on 4 article reviews

rabbit anti peg3 - by Bioz Stars, 2026-06

92/100 stars

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1) Product Images from "Diabetic uterus environment may play a key role in alterations of DNA methylation of several imprinted genes at mid-gestation in mice"

Article Title: Diabetic uterus environment may play a key role in alterations of DNA methylation of several imprinted genes at mid-gestation in mice

Journal: Reproductive Biology and Endocrinology : RB&E

doi: 10.1186/1477-7827-11-119

Figure Legend Snippet: Oligonucleotides and annealing temperature utilized for qAMP and qRT-PCR of imprinted genes

Techniques Used: Sequencing

Quantitative DNA methylation in DMRs of imprinted genes in 10.5dpc placentas as revealed by qAMP. Diabetic (n = 26 from 5 litters) and nondiabetic (n = 25 from 5 litters) placentas at mid-gestation were recovered. (A) The paternally methylated H19 ; (B, C) Percentage methylation values at single and groups of restriction sites in the maternally methylated DMRs of Snrpn and Peg3. The lines represent the DMRs which were analyzed and the letter indicates the recognition sites of the enzymes. M, McrBc; Hh, HhaI; Hp, HpaII. (D) Confirmation of gene methylation level was determined by qAMP to expected values. HhaI and McrBc sites in chr9:106724005–106724149 are unmethylated in the genome. A primer pair flanking these sites was utilized to amplify DNA (NIH 3 T3 mouse genomic DNA and CpG methylated NIH 3 T3 mouse genomic DNA, NEB) mixed at the ratios: 100:0, 75:25, 55:45, 50:50, 45:55, 0:100. The qAMP values were close to expected values. Data are presented as mean ± SE for each enzyme employed. White bar, non-diabetic group; black bar, diabetic group; *P < 0.05, **P < 0.01.

Figure Legend Snippet: Quantitative DNA methylation in DMRs of imprinted genes in 10.5dpc placentas as revealed by qAMP. Diabetic (n = 26 from 5 litters) and nondiabetic (n = 25 from 5 litters) placentas at mid-gestation were recovered. (A) The paternally methylated H19 ; (B, C) Percentage methylation values at single and groups of restriction sites in the maternally methylated DMRs of Snrpn and Peg3. The lines represent the DMRs which were analyzed and the letter indicates the recognition sites of the enzymes. M, McrBc; Hh, HhaI; Hp, HpaII. (D) Confirmation of gene methylation level was determined by qAMP to expected values. HhaI and McrBc sites in chr9:106724005–106724149 are unmethylated in the genome. A primer pair flanking these sites was utilized to amplify DNA (NIH 3 T3 mouse genomic DNA and CpG methylated NIH 3 T3 mouse genomic DNA, NEB) mixed at the ratios: 100:0, 75:25, 55:45, 50:50, 45:55, 0:100. The qAMP values were close to expected values. Data are presented as mean ± SE for each enzyme employed. White bar, non-diabetic group; black bar, diabetic group; *P < 0.05, **P < 0.01.

Techniques Used: DNA Methylation Assay, Methylation

Average DNA methylation levels in DMRs of imprinted genes in 10.5dpc fetus as revealed by qAMP. Diabetic (n = 12 from 4 litters) and control (n = 12 from 4 litters) fetus were collected at 10.5dpc of gestation. DNA was digested with Hha1 (Hh), HpaII (Hp) or McrBC (M) and amplified using real-time PCR. The locations of flanked restriction sites were displayed for each DMR. (A) Shown are the average methylation levels of paternally methylated gene H19 DMR; (B, C) represented the DNA methylation status in DMRs of Snrpn and Peg3 . Data were presented as mean ± SE for each enzyme employed. White bar, non-diabetic group; black bar, diabetic group.

Figure Legend Snippet: Average DNA methylation levels in DMRs of imprinted genes in 10.5dpc fetus as revealed by qAMP. Diabetic (n = 12 from 4 litters) and control (n = 12 from 4 litters) fetus were collected at 10.5dpc of gestation. DNA was digested with Hha1 (Hh), HpaII (Hp) or McrBC (M) and amplified using real-time PCR. The locations of flanked restriction sites were displayed for each DMR. (A) Shown are the average methylation levels of paternally methylated gene H19 DMR; (B, C) represented the DNA methylation status in DMRs of Snrpn and Peg3 . Data were presented as mean ± SE for each enzyme employed. White bar, non-diabetic group; black bar, diabetic group.

Techniques Used: DNA Methylation Assay, Amplification, Real-time Polymerase Chain Reaction, Methylation

Analysis of the mRNA and protein expression levels of imprinted genes in 10.5dpc placentas and fetus. (A, B) Placentas (n = 30 from 6 litters for each group) and fetus (n = 12 from 4 litters for each group) were collected at 10.5dpc of gestation. Total RNA was purified and reverse transferred into cDNA and then amplified using qRT-PCR. (A) Relative expression levels of H19, Snrpn and Peg3 in placentas. (B) Relative expression levels of H19, Snrpn and Peg3 in fetus. (C) The protein expression of Peg3 in placentas (n = 6) was investigated by western blot analysis and (D) the relative intensity of Peg3/beta-actin was evaluated by gel level analysis. (E) Placentas (n = 6) from diabetic and non-diabetic groups were stained by anti-Peg3 at 1:500 for histological analysis. Scale bar, 100 μm. Data are presented as mean ± SD. White bar, non-diabetic group; black bar, diabetic group; *P < 0.05, **P < 0.01.

Figure Legend Snippet: Analysis of the mRNA and protein expression levels of imprinted genes in 10.5dpc placentas and fetus. (A, B) Placentas (n = 30 from 6 litters for each group) and fetus (n = 12 from 4 litters for each group) were collected at 10.5dpc of gestation. Total RNA was purified and reverse transferred into cDNA and then amplified using qRT-PCR. (A) Relative expression levels of H19, Snrpn and Peg3 in placentas. (B) Relative expression levels of H19, Snrpn and Peg3 in fetus. (C) The protein expression of Peg3 in placentas (n = 6) was investigated by western blot analysis and (D) the relative intensity of Peg3/beta-actin was evaluated by gel level analysis. (E) Placentas (n = 6) from diabetic and non-diabetic groups were stained by anti-Peg3 at 1:500 for histological analysis. Scale bar, 100 μm. Data are presented as mean ± SD. White bar, non-diabetic group; black bar, diabetic group; *P < 0.05, **P < 0.01.

Techniques Used: Expressing, Purification, Amplification, Quantitative RT-PCR, Western Blot, Staining

The methylation level and expression of Peg3 in different litters. (A, B) the methylation level of Peg3 in different litters (n = 6 litters) at McrBc and HpaII was analyzed by qAMP; (C) the expression of Peg3 in s2, s3, s5 and s6 was evaluated by qRT-PCR. White bar, nondiabetic; black bar, diabetic; number and letter under X-axis, litter; *P < 0.05; **P < 0.01.

Figure Legend Snippet: The methylation level and expression of Peg3 in different litters. (A, B) the methylation level of Peg3 in different litters (n = 6 litters) at McrBc and HpaII was analyzed by qAMP; (C) the expression of Peg3 in s2, s3, s5 and s6 was evaluated by qRT-PCR. White bar, nondiabetic; black bar, diabetic; number and letter under X-axis, litter; *P < 0.05; **P < 0.01.

Techniques Used: Methylation, Expressing, Quantitative RT-PCR

DNA methylation and expression levels of Peg3 in placentas at mid-gestation. (A, B) Non-diabetic pronuclear embryos were transferred to normal/diabetic (NN/ND) pseudopregnant female and the DNA methylation and expression levels of Peg3 (n, NN:ND = 12:15) in 10.5d placentas were analyzed by qAMP and qRT-PCR, respectively. (A) methylation level of Peg3 in dpc10.5 placenta; (B) expression of Peg3 in placenta. (C, D) DN’s (n = 15 from 4 litters) and NN’s (n = 15 from 4 litters) placentas were collected at mid-gestation. DNA was digested with HpaII or McrBC and amplified using real-time PCR. (C) the methylation in DMRs of Peg3 in placentas; (D) Relative expression levels of Peg3 in placentas. Data were presented as mean ± SD. *P < 0.05, **P < 0.01.

Figure Legend Snippet: DNA methylation and expression levels of Peg3 in placentas at mid-gestation. (A, B) Non-diabetic pronuclear embryos were transferred to normal/diabetic (NN/ND) pseudopregnant female and the DNA methylation and expression levels of Peg3 (n, NN:ND = 12:15) in 10.5d placentas were analyzed by qAMP and qRT-PCR, respectively. (A) methylation level of Peg3 in dpc10.5 placenta; (B) expression of Peg3 in placenta. (C, D) DN’s (n = 15 from 4 litters) and NN’s (n = 15 from 4 litters) placentas were collected at mid-gestation. DNA was digested with HpaII or McrBC and amplified using real-time PCR. (C) the methylation in DMRs of Peg3 in placentas; (D) Relative expression levels of Peg3 in placentas. Data were presented as mean ± SD. *P < 0.05, **P < 0.01.

Techniques Used: DNA Methylation Assay, Expressing, Quantitative RT-PCR, Methylation, Amplification, Real-time Polymerase Chain Reaction

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Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Diabetic uterus environment may play a key role in alterations of DNA methylation of several imprinted genes at mid-gestation in mice

doi: 10.1186/1477-7827-11-119

Figure Lengend Snippet: Oligonucleotides and annealing temperature utilized for qAMP and qRT-PCR of imprinted genes

Article Snippet: The embedded placentas were sectioned at 8 μm and incubated with rabbit anti-Peg3 (1:500, Bioss, China, code: bs-1870R) overnight at 4°C.

Techniques: Sequencing

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Diabetic uterus environment may play a key role in alterations of DNA methylation of several imprinted genes at mid-gestation in mice

doi: 10.1186/1477-7827-11-119

Figure Lengend Snippet: Quantitative DNA methylation in DMRs of imprinted genes in 10.5dpc placentas as revealed by qAMP. Diabetic (n = 26 from 5 litters) and nondiabetic (n = 25 from 5 litters) placentas at mid-gestation were recovered. (A) The paternally methylated H19 ; (B, C) Percentage methylation values at single and groups of restriction sites in the maternally methylated DMRs of Snrpn and Peg3. The lines represent the DMRs which were analyzed and the letter indicates the recognition sites of the enzymes. M, McrBc; Hh, HhaI; Hp, HpaII. (D) Confirmation of gene methylation level was determined by qAMP to expected values. HhaI and McrBc sites in chr9:106724005–106724149 are unmethylated in the genome. A primer pair flanking these sites was utilized to amplify DNA (NIH 3 T3 mouse genomic DNA and CpG methylated NIH 3 T3 mouse genomic DNA, NEB) mixed at the ratios: 100:0, 75:25, 55:45, 50:50, 45:55, 0:100. The qAMP values were close to expected values. Data are presented as mean ± SE for each enzyme employed. White bar, non-diabetic group; black bar, diabetic group; *P < 0.05, **P < 0.01.

Article Snippet: The embedded placentas were sectioned at 8 μm and incubated with rabbit anti-Peg3 (1:500, Bioss, China, code: bs-1870R) overnight at 4°C.

Techniques: DNA Methylation Assay, Methylation

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Diabetic uterus environment may play a key role in alterations of DNA methylation of several imprinted genes at mid-gestation in mice

doi: 10.1186/1477-7827-11-119

Figure Lengend Snippet: Average DNA methylation levels in DMRs of imprinted genes in 10.5dpc fetus as revealed by qAMP. Diabetic (n = 12 from 4 litters) and control (n = 12 from 4 litters) fetus were collected at 10.5dpc of gestation. DNA was digested with Hha1 (Hh), HpaII (Hp) or McrBC (M) and amplified using real-time PCR. The locations of flanked restriction sites were displayed for each DMR. (A) Shown are the average methylation levels of paternally methylated gene H19 DMR; (B, C) represented the DNA methylation status in DMRs of Snrpn and Peg3 . Data were presented as mean ± SE for each enzyme employed. White bar, non-diabetic group; black bar, diabetic group.

Article Snippet: The embedded placentas were sectioned at 8 μm and incubated with rabbit anti-Peg3 (1:500, Bioss, China, code: bs-1870R) overnight at 4°C.

Techniques: DNA Methylation Assay, Amplification, Real-time Polymerase Chain Reaction, Methylation

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Diabetic uterus environment may play a key role in alterations of DNA methylation of several imprinted genes at mid-gestation in mice

doi: 10.1186/1477-7827-11-119

Figure Lengend Snippet: Analysis of the mRNA and protein expression levels of imprinted genes in 10.5dpc placentas and fetus. (A, B) Placentas (n = 30 from 6 litters for each group) and fetus (n = 12 from 4 litters for each group) were collected at 10.5dpc of gestation. Total RNA was purified and reverse transferred into cDNA and then amplified using qRT-PCR. (A) Relative expression levels of H19, Snrpn and Peg3 in placentas. (B) Relative expression levels of H19, Snrpn and Peg3 in fetus. (C) The protein expression of Peg3 in placentas (n = 6) was investigated by western blot analysis and (D) the relative intensity of Peg3/beta-actin was evaluated by gel level analysis. (E) Placentas (n = 6) from diabetic and non-diabetic groups were stained by anti-Peg3 at 1:500 for histological analysis. Scale bar, 100 μm. Data are presented as mean ± SD. White bar, non-diabetic group; black bar, diabetic group; *P < 0.05, **P < 0.01.

Article Snippet: The embedded placentas were sectioned at 8 μm and incubated with rabbit anti-Peg3 (1:500, Bioss, China, code: bs-1870R) overnight at 4°C.

Techniques: Expressing, Purification, Amplification, Quantitative RT-PCR, Western Blot, Staining

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Diabetic uterus environment may play a key role in alterations of DNA methylation of several imprinted genes at mid-gestation in mice

doi: 10.1186/1477-7827-11-119

Figure Lengend Snippet: The methylation level and expression of Peg3 in different litters. (A, B) the methylation level of Peg3 in different litters (n = 6 litters) at McrBc and HpaII was analyzed by qAMP; (C) the expression of Peg3 in s2, s3, s5 and s6 was evaluated by qRT-PCR. White bar, nondiabetic; black bar, diabetic; number and letter under X-axis, litter; *P < 0.05; **P < 0.01.

Article Snippet: The embedded placentas were sectioned at 8 μm and incubated with rabbit anti-Peg3 (1:500, Bioss, China, code: bs-1870R) overnight at 4°C.

Techniques: Methylation, Expressing, Quantitative RT-PCR

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Diabetic uterus environment may play a key role in alterations of DNA methylation of several imprinted genes at mid-gestation in mice

doi: 10.1186/1477-7827-11-119

Figure Lengend Snippet: DNA methylation and expression levels of Peg3 in placentas at mid-gestation. (A, B) Non-diabetic pronuclear embryos were transferred to normal/diabetic (NN/ND) pseudopregnant female and the DNA methylation and expression levels of Peg3 (n, NN:ND = 12:15) in 10.5d placentas were analyzed by qAMP and qRT-PCR, respectively. (A) methylation level of Peg3 in dpc10.5 placenta; (B) expression of Peg3 in placenta. (C, D) DN’s (n = 15 from 4 litters) and NN’s (n = 15 from 4 litters) placentas were collected at mid-gestation. DNA was digested with HpaII or McrBC and amplified using real-time PCR. (C) the methylation in DMRs of Peg3 in placentas; (D) Relative expression levels of Peg3 in placentas. Data were presented as mean ± SD. *P < 0.05, **P < 0.01.

Article Snippet: The embedded placentas were sectioned at 8 μm and incubated with rabbit anti-Peg3 (1:500, Bioss, China, code: bs-1870R) overnight at 4°C.

Techniques: DNA Methylation Assay, Expressing, Quantitative RT-PCR, Methylation, Amplification, Real-time Polymerase Chain Reaction

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